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Use of Nucleofector® Technology to Establish Stably Expressing Cell Lines
Amaxa GMBH
The number of stably expressing clones resulting from standard transfection experiments is often quite low, especially for cells which are difficult to transfect. This limits many research applications. We show here that the Nucleofector® Technology can successfully be used to generate stably expressing clones at high efficiency. Although a general protocol cannot be given, the data shown here can serve as a guideline for fast establishment of stable transfection protocols for various cell lines
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Efficient Delivery of Dharmacon SMARTpool siRNA Reagents in Hard-To-Transfect Cell Lines Using amaxa Nucleofector® 96-well Shuttle® System
Amaxa GMBH
In this Technical Note, Dharmacon and amaxa technologies were applied to Jurkat cells with the aim of performing future siRNA library screens. Jurkat cells are derived from a human acute T-cell leukemia line and are used extensively in the study of T cell signaling and cancer drug development. As with any suspension cell line, Jurkat cells are hard to transfect using reagentmediated delivery.
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The Nucleofector® 96-well Shuttle® System – Nucleofection® of Cell Lines and Primary Cells in an Automated Framework
Amaxa GMBH
Screening of siRNA or cDNA libraries requires an automated high-throughput transfection process. Besides mere throughput considerations, the main drivers for automation are the standardization and robustness of the process. Stability of all cell parameters is the key to statistically relevant data and valid results. Therefore we analyzed the stability of transfection results of different cell types during a time span typically needed to screen a focused siRNA library using Nucleofection®. The tr
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High-Throughput Nucleofection® of Primary Rat Hippocampal Neurons
Amaxa GMBH
Postmitotic neurons are routinely difficult to transfect. This application note describes the efficient, high-throughput Transfection® of plasmid DNA into primary hippocampal neurons. Using amaxa’s Nucleofector® 96-well Shuttle® System, 30 to 50 % of neurons were successfully transfected and showed normal neuronal development. In addition, quantitative down-regulation of target proteins by RNA interference was achieved.
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High Speed FRET based SNP Genotyping Measurement on the PHERAstar
BMG LABTECH, KBiosciences
Single Nucleotide Polymorphisms (SNP`s) have become an invaluable tool in the field of Genetic Research. Here we show the use of BMG LABTECH´s PHERAstar multimode HTS plate reader for high speed FRET based SNP Genotyping measurement. The new KASPar™ system, developed by KBiosciences, was used to assess the best performance of the PHERAstar. Optimized optical modules with dual emission lead to improvements in speed and accuracy of measurement.
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Increasing Chemist Productivity in an Open-Access Environment
ACD/Labs
“Open-Access” or “Walk-up” analytical instrumentation, has changed the way many routine analytical experiments are now run. Chemists, rather than analytical support groups, now carry the burden of processing, analysis, and interpretation of analytical data. This application note will highlight the benefits of having good NMR software tools that chemists can access, in the convenience of their own lab or office and how NMR prediction and verification algorithms can aid chemists in their interpre
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